How does pei transfection work

To obtain the best experience, we recommend you use a more up to date browser or turn off compatibility mode in Internet Explorer. In the meantime, to ensure continued support, we are displaying the site without styles and JavaScript. A simple and inexpensive method of condensing and linking plasmid DNA to carrier adenovirus particles is described.

The synthetic polycation polyethylenimine is used to condense plasmid DNA into positively charged nm complexes. These PEI—DNA complexes are then bound to adenovirus particles through charge interactions with negative domains on the viral hexon.

The resulting transfection complexes deliver plasmid DNA to cells by the adenovirus infectious route without interference from virus gene expression because psoralen-inactivated virus is employed.

You can also search for this author in PubMed Google Scholar. Reprints and Permissions. Baker, A. Polyethylenimine PEI is a simple, inexpensive and effective reagent for condensing and linking plasmid DNA to adenovirus for gene delivery. Gene Ther 4, — Download citation. Received : 08 January Accepted : 14 April Issue Date : 01 August Anyone you share the following link with will be able to read this content:.

Sorry, a shareable link is not currently available for this article. Provided by the Springer Nature SharedIt content-sharing initiative. Ready-to-use , Transporter TM 5 Transfection reagent is based on a proprietary polyethylenimine derivative.

The structure of Transporter TM 5 condenses DNA into positively charged complexes which enter the cell by endocytosis. Polyethylenimine PEI cellular transfection reagent Cellular transfection reagent. Linear polyethylenimine is a high-charge cationic polymer that readily binds highly anionic substrates. Industrially, linear PEI can improve the appearance of negatively charged dyes by modulating their properties and improving their adherence to surfaces.